Table 2.

Biochemical assays of various metabolites in gastrocnemius muscle and liver harvested from GF and conventionalized wild-type C57Bl/6J mice fed a Western diet

Metabolite,μmol/g proteinGastrocnemius muscleLiver
GFConventionalizedP valueGFConventionalizedP value
AMP6.14 ± 0.394.09 ± 0.420.0120.08 ± 0.8520.87 ± 1.600.68
ADP6.36 ± 0.897.19 ± 0.180.354.25 ± 0.465.19 ± 0.520.22
ATP26.78 ± 1.7928.56 ± 3.290.403.41 ± 0.734.13 ± 1.040.62
NAD+1.99 ± 0.061.88 ± 0.120.861.98 ± 0.181.15 ± 0.210.02
NADH0.06 ± 0.020.09 ± 0.030.270.29 ± 0.030.34 ± 0.060.46
  • Mean values ± SE are shown (n = 5).