Table S2.

Primers used in this study

PrimerSequence
spn_NotI_stopcodon_fwd5′-ATATGCGGCCGCTTATTTTACGTGTTCATATTTTG-3′
spn_NotI_fwd5′-ATATGCGGCCGCTTTTACGTGTTCATATTTTG-3′
spn_enterokinase_BamHI_rvs5′-CGGGATCCGACGATGACGACAAGAAAGTTTATTCTCAAAATGGAC-3′
spn_BamHI_rvs5′-CGGGATCCAAAGTTTATTCTCAAAATGGAC-3′
PSPIN_XbaI_fwd5′-ATCGTCTAGATTCTCTTTTACGTTACCAAAATATACATATAC-3′
PSPIN_KpnI_rvs5′-ATTAAGGTACCTAACTCCATCATAACACTGAATATTAAGAAAAT-3′
KO_Gibson_EcoRI_UP_fwd5′-GAGGCCCTTTCGTCTTCAAGAATTCCCTACAGAACTGCCTTTCAG-3′
KO_Gibson_KpnI_DN_rvs5′-GACTCTAGAGGATCCCCGGGTACCAAAAGATGGACATCACCAAG-3′
KO_UP_rvs5′-GCATTATTGAATATCTATCACTCCTCTAAAAATTG-3′
KO_DN_fwd5′-AGAGGAGTGATAGATATTCAATAATGCTTTGTAAC-3′
pLukM_spn_rvs5′-GCTACTAAAACCTTTTTAAATTTCATAGTTTCACTTTCTTTCTCTTTATA-3′
pLukM_spn_fwd5′-TATAAAGAGAAAGAAAGTGAAACTATGAAATTTAAAAAGGTTTTAGTAGC-3′
pCM29_pLukM_fwd5′-CATGCCTGCAGGTCGACTCTAGAAAACGCGCAGTTAATAAAAAG-3′
spn_pCM29_rvs5′-GAAACAGCTATGACATGATTACGAATTCTTATTTTACGTGTTCATATTTTG-3′
spn_qPCR_fwd5′-GATGATGCAAACTTCTTAGAACATGAATTAAGT-3′
spn_qPCR_rvs5′-GTCCTTTATCGGCGAAGTATGATCT-3′
spn_qPCR_probe5′-CTTGGTCAGCATTCTT-3′
agrA_qPCR_fwd5′-GTTACGAGTCACAGTGAACTTACCT-3′
agrA_qPCR_rvs5′-CGAGTTCTTAATTCAGCTGGATCATCT-3′
agrA_qPCR_probe5′-CATCGCTGCAACTTT-3′
gyrB_qPCR_fwd5′-CGCACGTACAGTGGTTGAAAA-3′
gyrB_qPCR_rvs5′-CGTGTTACTTCACGCGCTT-3′
gyrB_qPCR_probe5′-ACGTGCCGCCATAATA-3′
  • Restriction sites are underlined.