Table 1.

Metabolic rates during culture of SF188 cells with d-[1,6-13C2]glucose

MetaboliteRate
[1,6-13C2]glucose consumption220 ± 7
[3-13C]lactate production368 ± 6
[3-13C]alanine production40 ± 2
[4-13C]glutamate labeling (apparent)3.7 ± 0.2
Oxygen consumption68 ± 2
  • Cells were perfused with d-[1,6-13C2]glucose three times, including two coperfusions with l-[3-13C]glutamine (see text). These data are from one perfusion. Data in Figs. 1, 2A, and 3A are from the same experiment. All rates are in μmol per 109 cells per hr ± standard error.