Table 2.

Intracellular proteins that coimmunoprecipitate with the β2 subunit in brain

Gel bandProteinNCBI accession no.Mass, kDaNo. of unique peptidesMASCOT score*CI
1Actin β gi 49868 39.519220100
1Actin δ gi 809561 41.3321214100
2GAPDH gi 55153885 36.09147999.8
3Myelin basic protein 1 gi 69885032 21.515121100
3Myelin basic protein 3 gi 69885049 18.510149100
3Myelin basic protein 5 gi 69885065 17.23138899.9
4eEF-2 gi 13938072 96.220165100
5Pyruvate kinase 3 gi 16741633 58.4212134100
6GIRK1 (Kir3) gi 54037432 50.897096.7
7Clathrin HC gi 51259242 193.67439999.99
8Dynamin 1 gi 18093102 96.2122193100
9Tubulin α2 gi 80478451 50.824281100
10Cyclic nucleotide phophodiesterase 1 gi 14193680 45.0218177100
11Voltage-dependent anion channel 1 gi 38051979 32.06117297.3
12Spectrin α chain gi 94366114 286.757217100
13G-protein (Goα) gi 6754012 40.63118099.5
14Mitochondrial ATP synthase H + pump, β subunit gi 89574015 48.0517193100
15Neuronal nAChR, β2 subunit gi 31542397 57.871410199.9
16Creatine kinase gi 6753428 47.3722264100
17GPRIN1 gi 81892663 96.06188998.5
  • NIPs corresponding to gel band numbers in Fig. 2C are identified by protein accession number in NCBI, molecular mass (Mass), the number of unique sequenced peptides, a MASCOT protein score, and a protein confidence index (CI). Complete information on the sequence and mass of the digested peptides used to identify each protein is presented in SI Table 4.

  • *MASCOT minimal protein score = 70.

  • Derived from GPS Explorer.

  • Protein interactions validated by Western blotting.