Table 1.

Germ-line transformation in flies transgenic for attP and various φC31 integrase constructs

LineIntegrase constructDNA injected pUAS-lacZattB, ng/μlGiving w+ progeny
w+/total no.%
Set-up I
nos-φ-zh86Fb/attP-zh102DnosC31-nos21528/7040
nosNLS-zh86Fb/attP-zh102DnosC31NLS-nos21552/12243
vas-φ-zh86Fb/attP-zh102Dvas-φC31-vas21529/6942
vasNLS-zh86Fb/attP-zh102DvasC31NLS-vas21525/7732
vas-φ-zh86Fb/attP-zh102Dvas-φC31-vas80032/8040
Set-up II
attP-zh86Fb/nos-φ-zh102Dnos-φC31-nos21516/10316
attP-zh86Fb/nos-φNLS-zh102Dnos-φC31NLS-nos21549/14833
attP-zh86Fb/vas-φ-zh102Dvas-φC31-vas21547/9351
attP-zh86Fb/vas-φNLS-zh102Dvas-φC31NLS-vas21516/4933
attP-zh86Fb/vas-φ-zh102Dvas-φC31-vas80036/6655
  • The lines injected were homozygous for both an attP site and a φ C31 integrase construct. G0 adults (male and females) obtained after injection were individually crossed with y w animals and progeny were screened for white+ expression. Line designations indicate the origin of the integrase constructs and the free attP site (e.g., nos-φ-zh86Fb/attP-zh102D:nos-φC31 construct is located in the attP site of line ZH-attP-86Fb, and the free attP site is derived from line ZH-attP-102D). Because single G0 females generally exhibited a low fertility and transgenesis rate compared with males, in all subsequent transformation experiments two injected females were used for one y w outcross. However, although we still tested females for transgenic frequencies, we present in the Tables 2 and 3 only the transgenic values obtained from G0 males. The percentages indicate the fraction of all the fertile G0 adults that gave white+ offspring.