Table 3.

Comparison of targeting into various attP sites

ZH-attP lineattP positionG0 males giving w+ progenyEye color*
w+/total no.%
ZH-attP-2AX-2A0/770Orange
ZH-attP-22A2L-22A22/5639Light orange
ZH-attP-30A2L-30A23/5641Orange
ZH-attP-35B2L-35B21/5340Light orange
ZH-attP-36B2L-36B26/4657Orange
ZH-attP-51C2R-51C27/6442Light orange
ZH-attP-51D2R-51D43/7260Red
ZH-attP-58A2R-58A25/5149Light orange
ZH-attP-62B3L-62B26/6739Light orange
ZH-attP-64A3L-64A22/4154Orange
ZH-attP-68E3L-68E32/8537Light orange
ZH-attP-75C3L-75C19/5237Light orange
ZH-attP-86Fa3R-86F13/4628Red
ZH-attP-86Fb3R-86F40/9243Orange
ZH-attP-96E3R-96E28/6444Orange
ZH-attP-102D4–102D39/8645Red
  • Females homozygous for the vasC31 construct, located at 102D on the fourth chromosome, were crossed to males homozygous (or hemizygous) for an indicated attP site, and the offspring were injected with pUAS-lacZattB (215 ng/μl). Because line ZH-attP-2A has the attP site on the X chromosome, G0 males are devoid of an attP site and therefore are not expected to give rise to transgenic offspring.

  • *Eye colors were determined 3 days after eclosion (heterozygous state). All flies transgenic for the injected plasmid reveal a reddish punctate color at the inner side of each of the three ocelli; this additional phenotype served as a convenient confirmation for transgenic flies in the cases of light orange eye color.

  • Landing site ZH-attP-30A, though homozygous viable on its own, turned out to be homozygous lethal upon integration of UAS-lacZattB.