Ush1g^{+/−} | Ush1g^{−/−} | ||||

Geometric parameter | Mean ± SD | n | Mean ± SEM | n | Statistical comparison |

Projected height, tallest row | 5.8 ± 0.3 | 25 | 5.2 ± 0.2 | 58 | 0.12 |

Projected length, tallest row | 9.6 ± 0.7 | 25 | 8.5 ± 0.4 | 58 | 0.19 |

Interstereocilia distance, tallest row | 0.38 ± 0.04 | 25 | 0.33 ± 0.02 | 58 | 0.28 |

No. of stereocilia, tallest row | 29.5 ± 2.7 | 25 | 32.2 ± 3.1 | 58 | 0.50 |

No. of rows | 5.1 ± 0.2 | 24 | 4.7 ± 0.1 | 54 | 0.11 |

Distance between rows | 0.79 ± 0.05 | 24 | 0.74 ± 0.04 | 54 | 0.48 |

Apical cell surface area | 20.2 ± 3.2 | 25 | 24.6 ± 1.7 | 58 | 0.23 |

Measurements were based on scanning electron microscopy images of the utricle taken in

*Ush1g*^{−/−}mice (*n*= 10) and*Ush1g*^{+/−}mice (*n*= 5) on P8. After selection of a representative set of hair bundles, various geometric parameters were extracted using a custom interface allowing a semiautomatized segmentation of stereocilia. Projected heights of the tallest stereocilia row were evaluated under similar angles of view for the two mutants. Statistical comparisons using the Student’s*t*test did not reveal any significant difference in the hair bundle parameters between the*Ush1g*^{−/−}and*Ush1g*^{+/−}mice. Values are given as mean ± SEM. All lengths and distances are given in micrometers, and areas are given in square micrometers;*n*is the number of cells analyzed.