Table S1.

Comparative analysis of hair bundle geometric parameters in VHCs of Ush1g+/− and Ush1g−/− mice

Ush1g+/−Ush1g−/−
Geometric parameterMean ± SDnMean ± SEMnStatistical comparison
Projected height, tallest row5.8 ± 0.3255.2 ± 0.2580.12
Projected length, tallest row9.6 ± 0.7258.5 ± 0.4580.19
Interstereocilia distance, tallest row0.38 ± 0.04250.33 ± 0.02580.28
No. of stereocilia, tallest row29.5 ± 2.72532.2 ± 3.1580.50
No. of rows5.1 ± 0.2244.7 ± 0.1540.11
Distance between rows0.79 ± 0.05240.74 ± 0.04540.48
Apical cell surface area20.2 ± 3.22524.6 ± 1.7580.23
  • Measurements were based on scanning electron microscopy images of the utricle taken in Ush1g−/− mice (n = 10) and Ush1g+/− mice (n = 5) on P8. After selection of a representative set of hair bundles, various geometric parameters were extracted using a custom interface allowing a semiautomatized segmentation of stereocilia. Projected heights of the tallest stereocilia row were evaluated under similar angles of view for the two mutants. Statistical comparisons using the Student’s t test did not reveal any significant difference in the hair bundle parameters between the Ush1g−/− and Ush1g+/− mice. Values are given as mean ± SEM. All lengths and distances are given in micrometers, and areas are given in square micrometers; n is the number of cells analyzed.