Table 4.

Yield of active, disulfide bond-containing proteins expressed in the cytoplasm

StrainvtPA (+DsbC)Fab (1:2)PhoA
Wild type1 ± 0.51 ± 0.31 ± 0.2
Δgor ΔtrxB ahpC* 119.4 ± 10.9 11.5 ± 0.6
ΔgshB ΔtrxB ahpC*18.5 ± 2.85.0 ± 0.734.0 ± 2.0
Δgor ΔtrxB ΔkatG ahpC P166L31.1 ± 10.25.7 ± 0.1123.0 ± 5.0
ΔgshA ΔtrxB ahpC V164G32.6 ± 5.312.1 ± 1.5136.0 ± 13.0
ΔgshA ΔtrxB ahpC E171Ter10.3 ± 9.4 27.6 ± 4.0 158 ± 3.0
  • Results shown are a summary of those in Fig. S2. Yields are relative to those in wild-type (DHB4). Bold entries show the best oxidation for a given substrate.