Table 2.

PMF in aerobically growing and hypoxic nongrowing Mtb H37Rv

Drugs (μM)Δψ, mVΔpH, mVPMF, mV
Aerobic growing mycobacteriaNo drug66.2 ± 4.445.3 ± 5.5111.5 ± 9.9
Valinomycin (5)35.8 ± 2.335.8 ± 2.3
Nigericin (5)63.1 ± 4.063.1 ± 4.0
Rifampicin (1)62.7 ± 3.250.6 ± 3.2113.3 ± 6.4
Hypoxic nongrowing mycobacteriaNo drug73.1 ± 4.240.6 ± 9.2113.7 ± 13.4
Valinomycin (5)31.9 ± 5.331.9 ± 5.3
Nigericin (5)69.2 ± 10.869.2 ± 10.8
Rifampicin (1)65.1 ± 9.342.3 ± 8.6107.4 ± 17.9
  • The ΔΨ, ΔpH, and PMF were measured in aerobic growing and hypoxic nonreplicating Mtb maintained in Dubos medium (pH of 6.6). The experiment was carried out three times in triplicate; one of the representative experiment is shown.