Table 3.

Clozapine in combination with VPA increases nuclear DNA-demethylase activity in the mouse frontal cortex

Treatment3H3C-CG-reelin promoter demethylation, pmol per milligram of protein per h
Vehicle0.93 ± 0.063
VPA (0.5 mmol/kg, twice a day for 3 days)2.3 ± 0.29*
CLZ(15 μmol/kg, twice a day for 3 days)1.6 ± 0.074
VPA + CLZ5.3 ± 0.17**
  • Mouse FC nuclear extracts were incubated with 0.1nM of 3H3C-CG-reelin promoter (-693 to +141 bp) for 20 min. Each value is the mean ± SE of three mice. * P < 0.05 vs. vehicle; **, P < 0.01 vs. control (Student's t test).

  • The last injection of VPA+CLZ was administered 2 h before measurement.