Clozapine in combination with VPA† increases nuclear DNA-demethylase activity in the mouse frontal cortex
| Treatment | 3H3C-CG-reelin promoter demethylation, pmol per milligram of protein per h |
|---|---|
| Vehicle | 0.93 ± 0.063 |
| VPA (0.5 mmol/kg, twice a day for 3 days) | 2.3 ± 0.29* |
| CLZ(15 μmol/kg, twice a day for 3 days) | 1.6 ± 0.074 |
| VPA + CLZ | 5.3 ± 0.17** |
Mouse FC nuclear extracts were incubated with 0.1nM of 3H3C-CG-reelin promoter (-693 to +141 bp) for 20 min. Each value is the mean ± SE of three mice. * P < 0.05 vs. vehicle; **, P < 0.01 vs. control (Student's t test).
↵†The last injection of VPA+CLZ was administered 2 h before measurement.