Table S3.

GPR56-binding monobody affinity measurements by surface plasmon resonance

LigandAnalytekon (M−1 s−1) × 105koff (s−1) × 10−3Kd (nM) [koff/kon]
hECRβ14.129.371.5
hECRβ38.90.91.0
mECRβ34.610.823.2
hGAINβ35.40.140.3
mGAINβ39.25.86.3
β7hECR0.64.166.7
β7hPLL3.21.34.0
β12mECR0.82.227.9
β12mPLL1.91.15.6