Sample | Wells | No. negative wells | L | X | Y | LX | LY | XY | LXY* | All DNA^{†} | Short DNA^{‡} | Enrichment, % | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
X copies | Y copies | Fetal % | X copies | Y copies | Fetal % | (Short–all)/all | ||||||||||
M4176P | 376 | 285 | 0 | 57 | 7 | 25 | 1 | 1 | 0 | 94 | 9 | 18 | 63 | 8 | 23 | 28 |
M3848P | 384 | 295 | 0 | 54 | 6 | 26 | 1 | 2 | 0 | 92 | 9 | 18 | 61 | 8 | 24 | 31 |
M3960P | 2,681 | 2,196 | 0 | 361 | 21 | 96 | 2 | 3 | 2 | 507 | 28 | 11 | 391 | 26 | 13 | 19 |
M3146P | 383 | 310 | 0 | 57 | 5 | 10 | 0 | 0 | 1 | 75 | 6 | 15 | 62 | 6 | 18 | 19 |
M2098P | 2,271 | 1,768 | 0 | 339 | 22 | 134 | 2 | 4 | 2 | 538 | 30 | 11 | 372 | 28 | 14 | 33 |
L, extension product generated only by the long amplicon; X, extension product of ZFX; Y, extension product of ZFY.
↵*As these are maternal plasma samples, wells positive for LXY are assumed to contain one copy of LX (i.e., long X DNA molecule) and one copy of Y (i.e., short Y DNA molecule).
↵^{†}All DNA Y copies = {−ln(N − Y − LY − XY − LXY)/N]}N
All DNA X copies = {−ln(N − X − LX − XY − LXY)/N]}N
↵^{‡}Short DNA Y copies = {−ln(N − Y − XY − LXY)/N]}N
Short DNA X copies = {−ln(N − X − XY)/N]}N
where N = the total number of digital PCR wells analyzed and ln is the natural logarithm.