Table 1.

Cell fate selection statistics determined by using time-lapse microscopy

Cell fates
Infected cellDaughters of infected cell
ClassificationExample moviesPercentage of total (counts)
Daughter ADaughter BWild-type E. coli grown to stationary phasehflKE. coli mutant grown to exponential phase
Lysesn/an/aLytic eventS1, S255.6 (175)85.1 (382)
DividesLysesLysesLytic eventS4, S50.3 (1)1.3 (6)
DividesLysesDividesMixed fateS3, S6, S75.1 (16)1.6 (7)
DividesDividesDividesLysogenyS139.0 (123)12.0 (54)
Total100 (315)100 (449)
  • Bacterial cultures were infected at low API (phage-to-cell, ≈1:30) with λ Aam19 b::GFP cI857. The developmental outcome of infected (GFP-expressing) cells and, if applicable, their daughter cells, was monitored by time-lapse microscopy. The classification column outlines our mapping of developmental outcomes (lysis or division) of infected cells to overall cell fate outcomes. Cell fate statistics were collected for 2 conditions with different cell strains, growth media and growth phase at time of harvest. Example movies for the different combinations of cell fate outcomes are provided (Movies S1–S7). In some cases, the fate of an infected cell or one or both of its daughters could not be determined either because (i) the cell moved out of the field of view or became obscured by neighboring cells or (ii) the cell did not divide or lyse before the end of the time-lapse movie. Such ambiguous cases were not included in this table or in any analyses.