Table 2.

Enzyme kinetic parameters of WT and mutant EGFR kinases

KinaseKm[ATP], μMkcat, s−1kcat/Km[ATP],μM−1·s−1
WT5.2 ± 0.20.0265.00E-3
T790M5.9 ± 0.10.1372.32E-2
L858R148 ± 41.4841.00E-2
L858R/T790M8.4 ± 0.30.4565.43E-2