Table 1.

Nob1 binding to pre-18S rRNA fragments

RNA (5′ end / 3′ end)Kd, μM4
H45/18S (0)>25*
H45/A2 (+212)1.8 ± 0.5
H45/(+228)0.50 ± 0.10
H45/(+278)0.28 ± 0.05
    H45(−31)/A21.8 ± 0.5
H44(−164)/A20.24 ± 0.05
        H44Δ/A20.18 ± 0.05
  • Data are the averages of at least 3 experiments.

  • *Nob1 appears to bind this RNA as a dimer, so accurate comparisons to other data cannot be made.