Table 1.

Phage titer and survival of H2O2-induced lysogenic staphylococcal strains

Donor strain[H2O2], mMCFU*Phage titer
RN450NI8.4 × 108
0.057.1 × 108
0.12.9 × 108
0.51.8 × 108
16.3 × 107
RN10359NI5.9 × 1088.8 × 105
(RN450 lysogenic for 80α)0.051.2 × 1091.9 × 106
0.12.9 × 1079.0 × 107
0.51.8 × 1045.3 × 109
15.6 × 1032.0 × 108
JP3592NI6.7 × 108<10
(RN10359 cI G130E)0.051.8 × 108<10
0.11.9 × 108<10
0.51.8 × 108<10
10.8 × 108<10
RN451NI5.9 × 1082.8 × 104
(RN450 lysogenic for φ11)0.054.2 × 1084.9 × 104
0.14.9 × 1073.1 × 105
0.53.8 × 1052.8 × 106
15.6 × 1048.9 × 105
RN1030NIND<10
(RN451 recA-mutant)0.05ND<10
0.1ND<10
0.5ND<10
1ND<10
  • The means of results from 3 independent experiments are presented. Variation was within ± 5% in all cases. NI, not induced; ND, not determined.

  • *Number of cells recovered after 4 h of incubation.

  • Number of plaque-forming phages per milliliter of induced culture, using RN4220 as indicator strain.