Table 1.

Partial list of V-segment germline distribution in 100% nonredundant Kabat sequences (Kabat), scFv domain-specific amplicons (ampl.), scFv RCA, and Sanger sequencing from 1.7 × 104 nonredundant round 2 and 3 binders against 16 unique antigen targets (Pann.). Forty-eight of 51 IMGT functional HV germlines were recovered, 53 of 70 K/L germlines were recovered. (see Table S1 and S2 for complete list)

HVAmpl.RCAPann.KabatKVAmpl.RCAPann.Kabat
5–5117.2114.177.946.143–2027.9022.9814.557.32
1–699.9710.0012.515.701–3911.6911.1211.897.32
1–28.347.355.852.783–159.619.482.703.38
4–59/617.668.224.676.384–18.298.575.713.78
3–30/337.657.746.7510.101–56.347.007.402.63
3–236.096.398.3910.132–284.875.285.433.72
1–465.195.433.501.513–113.573.682.426.24
1–184.544.595.122.451–123.362.812.841.03
6–13.803.215.855.421–332.242.681.752.00
1–83.452.892.921.021–161.431.490.600.69
3–72.992.973.554.403-NL51.370.990.700.06
4–42.242.462.261.511–271.311.110.781.09
3–481.952.117.272.812–301.282.131.581.26
3–91.872.046.661.291–91.100.974.801.66
7–4–11.842.430.840.661–170.931.090.411.09
4–30–4/311.721.480.732.421–60.680.770.960.46
3–741.572.111.721.212–240.580.870.350.51
4–391.441.690.883.222–400.510.800.320.23
3–53/661.281.132.441.431–130.510.710.250.17
3–211.281.241.162.061D-160.420.230.090.23
3–111.101.210.721.956–210.390.490.340.11
3–151.021.531.352.862D-290.370.360.170.51
4-b0.841.393.050.833-NL10.280.230.030.00
1–30.761.071.011.951D-170.260.240.090.06
5-a0.570.510.261.871–80.250.380.180.57
2–700.540.830.220.443D-200.230.210.040.23
3–640.450.430.200.412–290.220.270.250.00
3–130.400.540.230.52LVAmpl.RCAPann.Kabat

3–490.380.460.260.666–571.621.776.020.11
1–580.310.350.080.223–211.362.056.385.26
3–720.290.370.150.331–401.001.194.424.92
3–730.250.320.111.432–141.001.133.749.21
1–240.210.310.120.301–440.821.034.743.78
2–50.180.290.291.273–190.660.620.532.80
3–430.160.170.170.083–10.570.690.634.23
3-h0.120.100.140.031–470.540.702.261.72
3–200.110.120.330.148–610.511.190.792.17
1–450.060.070.040.177–460.440.550.610.63
4–30–20.050.050.030.501–510.430.581.833.49
4–340.050.090.0510.817–430.300.560.261.20
4–280.040.060.000.1710–540.180.200.330.69
1-f0.030.050.100.172–230.130.130.071.26
2–260.010.030.060.252–80.080.110.182.52