Table 1.

Calpain gene disruption attempts

MethodPurposeOutcomeAttempts
Double cross-overGene knockout0 integrations6
Single cross-overGene recapitulation4 integrations4
Gene disruption0 integrations4
Allelic replacementSynonymous mutation7 mutations/33 clones1
Nonsynonymous mutation0 mutations/62 clones2