Table 1.

Metabolic profiling of Jurkat cells treated with rapamycin

% change (P < 0.05)
Amino acid
    Arginine42.2
    Phenylalanine33.7
    Histidine33.1
    Leucine/isoleucine23.4
    Tyrosine23.1
    Proline22.9
    Methionine21.4
    Valine19.7
    Tryptophan16.3
    Glutamic acid5.9
    Glutamine6.6
    Hydroxyproline23.2
    Threonine26.2
    Homoserine28
    Taurine30.7
    Serine30.7
    Asparagine31.3
Mitochondrial metabolism
    Fumaric acid/maleic acid19.9
    Malic acid28.5
    Alpha-keto-glutarate30.4
    Succinic acid37.1
    Fumaric acid/maleic acid46.3
    Orotic acid99.6
    Acetyl-CoA57
    Malonyl-CoA47
Glucose metabolism
    F1P/F6P/G1P/G6P85.3
    Ribose-5-P/ribulose-5-P49.2
    Lactic acid40.8
    UDP-glucose27.7
Lipid metabolism
    Glycerol55.2
    Carnitine26.1
Nucleotides
    ATP66.3
    UTP125.5
    CDP70.1
    GTP78.1
    UDP86.6
    CTP134
    GDP52
    ADP79
  • Jurkat cells were treated with 100 nM rapamycin for 25 min. Intracellular metabolites were extracted and profiled using LC-MS/MS. The percent changes in relative levels of peak areas of metabolites that differed significantly (P < 0.05) after rapamycin treatment are displayed. Profiling experiments were performed in 5 biological replicates. Bold indicates an increase, and italic indicates a decrease, in relative levels.