Table 1.

Metabolic profiling of Jurkat cells treated with rapamycin

% change (P < 0.05)
Amino acid
    Glutamic acid5.9
Mitochondrial metabolism
    Fumaric acid/maleic acid19.9
    Malic acid28.5
    Succinic acid37.1
    Fumaric acid/maleic acid46.3
    Orotic acid99.6
Glucose metabolism
    Lactic acid40.8
Lipid metabolism
  • Jurkat cells were treated with 100 nM rapamycin for 25 min. Intracellular metabolites were extracted and profiled using LC-MS/MS. The percent changes in relative levels of peak areas of metabolites that differed significantly (P < 0.05) after rapamycin treatment are displayed. Profiling experiments were performed in 5 biological replicates. Bold indicates an increase, and italic indicates a decrease, in relative levels.