Table 1.

Effect of ARNT PAS-B mutations on relative populations in conformations

MutationPercentage 1Percentage 2
Wild type>99<1
F444Q>99<1
F444Q/I454A/Y456T>98<2
F444Q/I454F/Y456T>98<2
F444Q/Y456T/I458E>98<2
Y456A>98<2
Y456S8119
Y456T5050
P449A/Y456T3268
F444Q/T445A/Y456T3169
F444Q/Y456T1981
F444Q/Y456T/T460V1585
F444Q/T445Y/Y456T1486
F444Q/N448T/Y456T1090
F444Q/N448W/Y456T<2>98
F444Q/Y456T/I457F<2>98
F444Q/F446A/Y456T<1>99
  • For each species, relative populations of conformers 1 and 2 were established by 15N-1H HSQC measurements and comparisons of peak intensities at 10–18 pairs of cross-peaks. Each measurement carries ≈8% relative error on any given ratio.