Table 1.

Mutator effects of pol3-R696W, pol3-D762A,D764A, and pol3ΔKanMX alleles in haploid and diploid cells

GenotypepPOL3Canr mutation (x 10−7)*
POL3+2.4 (2.2–3.2)
pol3-R696W+67 (60–73)
pol3-R696W rev3+58 (45–89)
pol3ΔKanMX+6.7 (5.4–17)
pol3-D762A,D764A+7.6 (6.9–9.1)
POL3/POL3<0.3
POL3/pol3-R696W<0.3
POL3/POL3+<0.3
POL3/pol3-R696W+<0.3
pol3-R696W/pol3-R696W (1)†+69 (56–90)
pol3-R696W/pol3-R696W (2)†+20 (13–28)
pol3-R696W/pol3-R696W rev3/rev3+41 (36–51)
pol3ΔKanMX/pol3-R696W+5.4 (4.2–6.0)
  • *The values are medians for at least nine cultures. The 95% confidence limits are given in parentheses.

  • pol3-R696W/pol3-R696W (1) and (2) were constructed using PSD93 and E134×1B-D770 strains, respectively.