Table 1.

E. coli strains and plasmids used in this study

Strain or plasmidRelevant genotype or propertySource
DFB228fliM-null strain(30)
RP3098flhDC deletion strain; expresses no chromosomal flagellar genesGift from J.S. Parkinson
pTBM30Ptac expression vector; Apr(31)
pHT100GST-only expression vector; Kmr(30)
pHT96GST-FliN expression vector; Kmr(30)
pKP235FliM1–34CheY fusion in pTBM30 expression vector; AprThis study
pKP453FliM1–34CheY(D57A) in pTBM30 expression vector; AprThis study