Table 4.

Effect of Rubisco mutations on growth and H2 production

StrainGenotypeg (h)H2 yield (mol × mol ace−1)Sp. H2 productivity (mmol × g DCW−1 × h−1) H2:biomass (mol × g protein−1)
CGA009Wild type7.4 ± 0.2None detected
CGA669ΔcbbLS::KmR ΔcbbM No growth
CGA676 nifA* 8.3 ± 0.80.43 ± 0.041.12 ± 0.0866 ± 5
CGA679 nifA* ΔcbbLS::KmR ΔcbbM 12.8 ± 0.8 0.58 ± 0.03 1.02 ± 0.0792 ± 3
CGA679 complemented nifA* ΔcbbLS::KmR ΔcbbM (pBBPcbbLSX)9.8 ± 1.40.44 ± 0.080.98 ± 0.0468 ± 11
  • Cultures were grown in minimal medium with 20 mM sodium acetate and NH4 + as the nitrogen source. Values are averages ± SDs from 3 to 5 biological replicates.

  • Specific H2 productivity. Determined using the Monod model as described in ref. 21.

  • Significantly different from CGA676 characteristic (Student's t test, two-tailed, equal variance, P < 0.05).