Table 1.

Effect of PAI mutation/marker location on frequency of transfer

Donor strainInsertion site/genotypeTransfer frequency (per donor)
V583int::pJMM43Integrase gene2.9 (± 1.9) × 10−10
V583cbh::pJMM33Conjugated-bile salt hydrolase5.4 (± 3.8) × 10−10
V583ef0592::pJMM40Large cell-wall associated protein6.1 (± 2.1) × 10−10
V583cbh::pJMM33*Conjugated-bile salt hydrolase2.5 (± 2.0) × 10−10
V583cbh::pJMM33ΔexcConjugated-bile salt hydrolase, deleted excisionase8.1 (± 1.7) × 10−10
V583cbh::pJMM33ΔoriTConjugated-bile salt hydrolase, deleted oriT2.9 (± 4.1) × 10−10
V583cbh::pJMM33Δef0503Conjugated-bile salt hydrolase, deleted traG1.9 (± 2.2) × 10−10
  • *In the presence of 300 μg/mL of DNase, see SI Text.