Table 3.

Kinetic parameters for the wild-type and mutant enzymes

EnzymeKM, units in μMkcat, units in 1/skcat/KM (fold decrease)
Sa (WT)14.5 ± 3.5312.14 (1.00)
Sa (V31Y, F92I)43 ± 2.62.80.06 (36)
Sa (V31Y, F92S)58 ± 3.01.40.02 (107)
Sa (V31F, F92L)45 ± 4.30.310.007 (306)