Table 2.

The fitness of the sHLA peptides sequences to the HLA consensus binding motifs of the donors

SubjectHLA allele% high-certainty peptidesHLA-A+B supertype
Healthy 1; 250 high-certainty peptidesHLA-A0137A01
HLA-A0212A02
HLA-B35HLA-Cw0449B07*
Unassigned motif2
Healthy 6; 180 high-certainty peptidesHLA-A0215A02
HLA-A6848A03
HLA-B1824B44
HLA-B55HLA-Cw077B07
HLA-Cw034*
Unassigned motif2
Healthy 4; 200 high-certainty peptidesHLA-A24HLA-Cw0742A24
HLA-A6830A03
HLA-B4912B44
HLA-Cw017*
Unassigned motif (HLA-B22)12
AML-1; 330 high-certainty peptidesHLA-A0333A03
HLA-B276B27
HLA-B35HLA-Cw0448B07*
Unassigned motif (HLA-Cw1502)13
Healthy 5; 244 high-certainty peptidesHLA-A2617A01
HLA-A6614A03
HLA-B384B27
HLA-B5144B07
Unassigned motif (HLA-Cw12, HLA-Cw14)21
MM-1; 250 high-certainty peptidesHLA-A0212A02
HLA-A1133A03
HLA-B5120B07
HLA-B525B62
Unassigned motif (HLA-Cw02, HLA-Cw12)30
MM-2; 158 high-certainty peptidesHLA-A1134A03
HLA-A2613A01
HLA-B078B07
HLA-B381B27
Unassigned motif (HLA-Cw12, HLA-Cw15)43

Only sHLA peptidomes with above 150 high-certainty identified peptides were selected for this analysis. Fitness to the consensus binding motifs was assigned according to the HLA A and HLA B supertypes (22) and to HLA Cw (*) according to (44).