Table 1.

The role of bacteria in diet-induced mating preference of D. melanogaster

ExperimentFly treatment*MatingsSII, mean ± SEMP value
1Starch-grown × CMY-grown180.27 ± 0.02<0.0001
2Experiment 1 after antibiotics100.01 ± 0.030.4483
3Experiment 2 after infection with homologous bacteria40.22 ± 0.030.0024
4Experiment 3 with Lactobacillus replacing homologous bacteria in starch-bred flies40.16 ± 0.060.0392
5Experiment 3 with Lactobacillus plantarum replacing homologous bacteria in starch-bred flies50.19 ± 0.050.0004
6Infection control (no added bacteria)4−0.04 ± 0.080.4052
  • *After all treatments, the flies were grown for one generation in CMY medium before performing the mating preference test.

  • P value of the normal approximation to the binomial test. P < 0.05 was considered to indicate significant mating preference.

  • Antibiotic-treated starch- and CMY-grown flies were infected with bacteria isolated from their respective growth medium (before antibiotic treatment).