Table 1.

Mutations found in HPRT cDNAs prepared from 6-TG–resistant colonies of T47D, HCT116, and MDA-MB-231 cells

Cell linesTreatmentNumber of clones analyzedClones without mutation in the coding sequenceClones with frameshift mutation(s)Clones with deletion(s)Clones with only a single deletionClones with one or more exons lackingClones with other types of deletionsClones with insertion(s)Clones with transition(s)Clones with transversion(s)
T47DMock240121417244140
Del ex. 3: 11Del 1: 7ins 1: 1Transitions: 5
Del ex. 2+3: 9
Del ex. 4+5: 4
T47DLSMCM4002040253811371
Del l ex. 3: 14Del 17: 3Ins 1: 3Transitions: 7Transitions: 3
Del ex. 2+3: 16
Del ex. 4 + 5: 5
Del ex. 2+3+4: 1Del 1: 21
Del ex. 6+7+8: 1
Del ex. 5: 1
HCT116− Doxycycline14113130013402
Del 2: 1Ins 1: 5Transitions: 7Transversions: 3
Del 1: 12
HCT116− Doxycycline14113130013402
Del 2: 1Ins 1: 5Transversions: 2
Del 1: 12
HCT116+ Doxycycline25418110291366
Del ex. 3: 1Del 1: 16Ins 1: 17Transitions: 6Transversions: 8
Del ex. 2+3: 1
MDA-MB-231− Doxycycline113810583010
Del ex. 2+3: 1Del 1: 5Transitions: 1
Del ex. 7: 8
MDA-MB-231+ Doxycycline242172111179042
Del ex. 2: 1Del 1: 17Transitions: 6Transversions: 2
Del ex. 2 + 3: 1
Del ex. 7: 16
  • Ex., exon; LSMCM, LPS-stimulated macrophage-conditioned medium; Mock, unstimulated macrophage-conditioned medium.