Table 1.

The secreted FFA and intracellular FFA of SD strains

Strains*Doubling time, HourFinal cell density, cells/mLFFA-secretion yields,§ mg/LSecreted FFA, mg/cellIntracellular lipids/FFA, mg/cellGenetic modifications
SD1007.43.2 × 1091.8 ± 0.060.05 × 10-110.16 × 10-11wild type
SD21611.98.3 × 10883.6 ± 8.38.0 × 10-113.01 × 10-11‘tesA overexpression and aas deletion (Δslr1609PpsbA2 ‘tesA)
SD22512.18.3 × 10883.6 ± 11.48.0 × 10-112.38 × 10-11PHB synthesis gene deletion ACC overproduction Δ(slr1993-slr1994)∷ Pcpc accBC Prbc accDA)
SD23212.47.5 × 10890.5 ± 6.49.4 × 10-111.89 × 10-11sll1951 deletion C10∶0 C12∶0 TEs (Δsll1951Embedded Image Uc fatB1 Prbc Ch fatB2)
SD24314.31.0 × 10992.9 ± 3.010.2 × 10-111.24 × 10-11cyanophycin synthesis gene deletion C8∶0 C10∶0 TE [Δ(slr2001-slr2002)∷ Embedded Image Ch fatB2]
SD24916.71.3 × 109146 ± 2114.3 × 10-111.05 × 10-11PBP2 gene deletion C14∶0 TE (Δslr1710Embedded Image Cc fatB1)
SD27714.11.0 × 109197 ± 1420.0 × 10-111.12 × 10-11phosphotransacetylase gene deletion and codon-optimized tesA137 gene (Δslr2132Ptrc tesA137)
  • *The genotypes and constructions of strains were described in Table S1.

  • The doubling times were determined for exponential growing cultures (density below 108 cells/mL) in separate experiments.

  • The final cell density was determined by cfu.

  • §Twenty milliliter cultures were extracted by hexane and analyzed by GC for FFA amounts.

  • For each strain, the secreted FFA per cell was calculated based on the final cell density and the FFA-secretion yield of a single culture.

  • The intracellular unsecreted FFAs were extracted by the Folch method and calculated concentrations based on final cell densities.