Table 1.

Provisional sequencing model for de novo assembly

Libraries, insert types*Fragment size, bpRead length, basesSequence coverage, ×Required
Fragment180≥10045Yes
Short jump3,000≥100 preferable45Yes
Long jump6,000≥100 preferable5No
Fosmid jump40,000≥261No
  • *Inserts are sequenced from both ends, to provide the specified coverage.

  • More generally, the inserts for the fragment libraries should be equal to ~1.8 times the sequencing read length. In this way, the reads from the two ends overlap by ~20% and can be merged to create a single longer read. The current sequencing read length is ~100 bases.

  • Long and Fosmid jumps are a recommended option to create greater continuity.