Reduced T cell population in thymus, spleen, and peripheral blood from mutant Atm homozygous mice

TissueGenotypeThy1CD3αβTCRCD4CD8CD4+CD8CD4CD8+CD4+CD8+
ThymusControl98.8  ±  0.620.3  ±  1.518.0  ±  1.72.8  ±  1.612.1  ±  0.58.9  ±  1.678.0  ±  2.3
Atm98.5  ±  0.45.0  ±  1.3*5.4  ±  1.3*2.9  ±  0.93.6  ±  0.9*4.6  ±  1.488.9  ±  3.0**
SpleenControl33.7  ±  1.741.6  ±  1.444.7  ±  1.760.2  ±  1.925.0  ±  1.614.5  ±  0.70.2  ±  0.1
Atm10.0  ±  1.5*15.6  ±  1.9*20.0  ±  1.8*85.5  ±  1.8*8.8  ±  1.2*5.4  ±  1.4*0.2  ±  0.1
BloodControl39.7  ±  4.675.4  ±  3.1ND28.3  ±  2.154.9  ±  1.716.6  ±  0.80.2  ±  0.1
Atm15.9*34.6*ND45.1**37.3*17.60.1
  • Shown are the percentage of cells staining positive for each surface molecule by flow cytometry in Atm homozygotes and control (Atm heterozygote and wild-type) mice. n = 3 in all samples except in ATM blood, where n = 2. ND, not determined. ∗, P < 0.01; ∗∗ 0.01 < P < 0.05 by Student’s t test analysis.