Effects of T channel antagonists on the zona pellucida-induced acrosome reaction

TreatmentConc.Acrosome reacted, %
+Control+ZP
Medium23  ±  862 ± 6*
Pimozide0.1 μM21  ±  349 ± 5*
1.0 μM18  ±  627 ± 4
Amiloride0.1 mM17  ±  454 ± 7*
0.5 mM22  ±  333 ± 4*
  • Capacitated mouse sperm were incubated for 10 min in culture medium or in medium supplemented with the indicated T channel antagonists. The fraction of acrosome reacted sperm in culture medium-treated populations was 19%  ±  5%, and this background value was not altered significantly by treatment with T channel antagonists (range of means for pimozide and amiloride, 16–20%). Sperm were incubated an additional 20 min after addition of control glycoproteins (+Control, 50 μg/ml transferrin or fetuin) or soluble extracts of zonae pellucidae (+ZP, 50 μg/ml) were added and acrosome reactions were assessed. The results are given as the mean (±SEM) of triplicate experiments, with triplicate samples obtained in each experiment and with 100–200 sperm assayed per sample. 

  • Sperm treated with zonae pellucidae differ from sperm treated with control glycoproteins (P < 0.01). 

  • Sperm treated with T channel antagonists differ from culture medium controls (P < 0.01).