Time course of β-gal expression following a single injection of AAV-lacZ

Time, weeksβ-gal expression, ng/muscle% cross-sectional area expressing β-gal
 21441  ±  45818  ±  6
 4951  ±  17620  ±  4
 8839  ±  43624  ±  3
121878  ±  52129  ±  5
242579  ±  116522  ±  3
321242  ±  48424  ±  3
  • The left and right tibialis anterior muscles were injected with 8 × 109 vector particles of AAV-lacZ. One member of the pair of injected muscles was processed for β-gal expression (n = 5, ±SEM). The other member was processed for histochemical detection of β-gal and determination of the cross-sectional area of the tibialis anterior expressing β-gal. Mean cross sectional areas ± SEM are shown.