Effects of antisense deoxyoligonucleotides on termination of transcription from B. subtilis pyr templates in vitro

Oligo added% termination
−PyrR, −UMP+PyrR, +UMP−PyrR, −UMP
[Oligo]/[Template]
101001000
Template 1
 None32  ±  1291  ±  5
 T11353746
 T12353213
Template 2
 None67  ±  494  ±  3
 AT21719096
 AT22626267
 T23636367
 T2460447
 BL2261234
 BL23666764
 BL2459307
 BL25676871
 BL27676645
 BL28676115
 BL23 + BL25NDND41
  • Oligo, oligonucleotide; ND, not determined. The numbers 10, 100, and 1000 give the molar ratio of deoxyoligonucleotide to DNA template in the experiment.