Characterization of normal and tumor-derived breast epithelial cells

Cell linesCell typesEstrogen receptor (31)p53Cyclin E (31, 32)pRb*
 1. 70NN-mortal+  (39)++
 2. 81NN-mortal+  (39)++
 3. 76NN-mortal+  (39)++
 4. MCF-10-AN-immortalized+  (40)++
 5. MCF-7A (pe)++  (40)++++
 6. MDA-MB-157C (pe)−  (40)+++++++
 7. MDA-MB-231A (pe)−  (40)+++++
 8. MDA-MB-436A−  (41)+++++
 9. T47DDC (pe)+−  (40)+++
10. BT20C++  (40)+++
11. HBL-100T (bm) SV40  transformed−  (40)+++
12. HS-578TDC−  (40)++++
13. ZR75TIDC++  (40)++++
  • Cell type, estrogen receptor (ER), p53, and cyclin E status as determined in indicated references. +, wild type; ++(++++), varying degrees of overexpression with MDA-MB-157 showing the highest degree (64-fold, hence 6 + s) of cyclin E overexpression. N, normal breast cells from reduction mammoplasty; A, adenocarcinoma; pe, pleural effusion; C, carcinoma; DC, ductal carcinoma; T(bm), tumor breast milk; SV40, simian virus 40; IDC, infiltrating DC; −, mutant or not expressed. 

  • pRb status is adopted from Fig. 1, in which + indicates wild type and present in hypo- and hyperphosphorylated forms, and − indicates mutated or virally bound and inactive.