Table 2

Serum IgE responses induced by intranasal immunization with OVA and either mCT S61F or nCT as mucosal adjuvants

Treatment* groupTotal IgE, ng/mlAg-specific IgE, reciprocal log2 titer
OVACT-B
OVA alone186  ± 1083.99  ± 1.25<3
OVA + rCT-B159  ± 833.63  ± 0.51<3
OVA + nCT1,094  ± 2379.14  ± 0.796.22  ± 0.84
OVA + S61F768  ± 2188.10  ± 1.054.24  ± 0.88
  • * Mice were immunized as described. Each group contained five mice, and the results are representative of three separate experiments. 

  • Serum IgE levels reached maximum levels at 2 weeks following the initial immunization and were determined by ELISA (for total IgE) or by IgE-capture luminometric assay (for Ag-specific IgE). 

  • Significantly lower when compared with nCT (P < 0.05).