Yeast and bacterial strains

StrainGenotypeUse (source)
Yeast
 Y800 MAT a  leu2-Δ98 cry1R ade2-101  HIS3 Mutagenesis of ARP100
MATα leu2-Δ98 CRY1 ade2-101 his3-Δ200
ura3-52 can1R lys2-801 CYH2 trp1-1
ura3-52 CAN1 lys2-801 cyh2R TRP1  Ciro
 Y800-T1aMATa ade2-1 leu2-Δ98 ura3-52 lys2-801 trp1-1 his3-Δ200 CiroMutagenesis of SPA2
 W303-1B MATα ura3-1 ade2-1 trp1-1 his3-11,15 leu2-3,112 can1-100 Mutagenesis of SER1 (R. Rothstein, Columbia University)
 Y1279 MAT a  leu2-Δ98 ade2-101 his3-Δ200 Mutagenesis of BDF1 (B. Santos, Yale University)
MATα LEU2  ade2-101 HIS3
ura3-52 trp1-901 lys2-801
ura3-52 trp1-901 lys2-801
Bacteria
 B224/RDP146FrecA1 (Δlac-pro) rpsERecipient for F factor in mating (2)
 B211B224 plus pLB101 (pACYC184 with tnpA)Constitutively expresses Tn3 transposase (2)
 B425NG135 (K12 recA56 gal-ΔS165 strA) plus pNG54 (pACYC184 with tnpR)Constitutively expresses Tn3 resolvase (13)
 B426B224 plus pOX38::mTn-4xHA/lacZTransposon mutagenesis
 B427B224 plus pOX38::mTn-3xHA/lacZTransposon mutagenesis
 B428B224 plus pOX38::mTn-3xHA/GFPTransposon mutagenesis
 DH5αendA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF) U169 deoR [φ80 dlacΔ(lacZ)M15]Cloning, plasmid preparation