Differentiation of LS857 cells by thiazolidinediones and RXR-specific retinoids leads to cell cycle withdrawal

ControlPIO/268 1PIO/268 2
Cells counted500510595
BrdUrd + (%)232  (46)173  (34)156  (26)
Lipid + (%)0204  (40)233  (51)
BrdUrd + lipid + (%)NA22  (4)17  (3)
  • LS857 cells were cultured in the presence or absence of pioglitazone. Following induction of morphologic differentiation, pioglitazone was withdrawn. After 48 hr of continued culture in the absence of pioglitazone, cells were labeled for 48 hr with BrdUrd, stained, and visualized microscopically. NA, not available.