Table 1

Adenovirus E4 ORF1 transforming proteins possess consensus PDZ domain-binding motifs at their C termini*

PDZ domain-binding motifPosition
 −3  −2  −1  0
Transforming activityX(S/T)X(V/I)
9ORF1+++++ATLV
 mutIII-AAP
 mutIII-BATLVSACFL
 mutIII-C+DTLV
 mutIII-D+ATPV
 T123ANDAALV
 T123DNDADLV
 V125ANDATLA
12ORF1+++++ASLI
3ORF1+++++ATMI
5ORF1+++++ASNV
APCN/AVTSV
  • * The four C-terminal amino acid residues of each wild-type protein are shown. Alterations of mutant 9ORF1 proteins are depicted as outlined amino acid residues. 

  • In vitro transforming activities of wild-type and mutant E4 ORF1 proteins (7, 15). +++++, strong transforming activity; +, weak transforming activity; −, no detectable transforming activity; ND, not done; N/A, not applicable.