Table 1

Viability of tif4631 and tif4632 mutants

GeneStrainGeneration time, hr Pab1p binding
TIF4631 YAS20692.02.0Yes
tif4631-ΔN187 YAS20702.02.4Yes
tif4631-ΔN300 YAS20712.44.8No
tif4631-ΔN400 YAS20762.44.5No
tif4631-ΔN500 DeadDeadNo
tif4631-213 YAS20752.42.9No
tif4631-459 YAS20744.1DeadND
tif4631-ΔN187,459 YAS21042.1DeadND
tif4631-ΔN300,459 DeadDeadND
TIF4632 YAS19812.02.0Yes
tif4632-ΔN200 YAS19832.02.2Yes
tif4632-ΔN300 YAS19842.32.6No
tif4632-ΔN400 YAS19852.43.4No
tif4632-ΔN500 DeadDeadNo
tif4632-233 YAS20012.42.4No
  • Yeast strain YAS1948 (a ade2 his3 leu2 trp1 ura3 pep4::HIS3 tif4631::LEU2 tif4632::ura3 pTIF4632URA3CEN) or YAS1947 (α ade2 his3 leu2 trp1 ura3 tif4631::LEU2 tif4632::ura3 pTIF4632URA3CEN) were transformed with TRP1CEN plasmids (6) bearing the indicated tif4631 or tif4632 genes, respectively. Lack of growth on 5-fluoroorotic acid plates after 2 weeks was indicative of no viability. Growth rates were measured in yeast extract/peptone/dextrose (YPD) medium at the indicated temperatures. In vitro Pab1p-binding data are summarized from previous data (6) and from Fig. 1. ND, not determined.