Table 1

Bacterial strains used in this study

StrainRelevant genotype*Ref. or source
S. typhimurium
 LT2Wild typeJ. Roth
 AK3042zjj-3042::Tn10dTet linked 99% to serB+ 22
 AK3112zjj-3112::Tn10dTet linked 78% to serB+ 22
 SA965leuBCD39 ara-7 HfrK17(pro-metA-ilv … purE) 23
 TE958leuA414(Am) hsdL (rm+) serB1465::MudJ 24
 TT460pyrB692::Tn10 25
 TT2661zcc-7::Tn10 linked 80% to put610 26
 TT8371thr-469::MudA 24
 TT16813recD542::Tn10dCam 27
 MST1503zcc-5::Tn10 linked 50% to putP1123 26
 MST1504zcc-4::Tn10 linked 20% to putA1146 28
 MST1933mutS121::Tn10 29
 MST3063leuA414 (Am) hsdL (rm+) FelsmutS121::Tn10This study
 MST3433ΔmutS280 recD542::Tn10dCamThis study
 MST3434ΔmutS281This study
 MST3516zjj-3042::Tn10dTet serB1465:MudJThis study
 MST3517zjj-3112::Tn10dTet serB1465::MudJThis study
S. typhi
 Ty2Wild typeG. Mora
 TYT1148recD542::Tn10dCamThis study
 TYT1258ΔmutS153This study
 TYT1512ΔmutS153 recD542::Tn10dCamThis study
 TYT1513serB1465::MudJThis study
 TYT1681serB1465::MudJ thr-469::MudAThis study
 TYT1685serB1465::MudJ thr-469::MudA pyrB692::Tn10This study
 TYT1932Hybrid (thr-pyrB substitution from S. typhimurium)This study
 TYT1933ΔmutS284 hybrid (thr-pyrB substitution from S. typhimurium)This study
 TYT1934recD542::Tn10dCam hybrid (thr-pyrB substitution from S. typhimurium)This study
 TYT1935ΔmutS284 recD542::Tn10dCam hybrid (thr-pyrB substitution from S. typhimurium)This study
  • * Tn10dTet refers to the transposition-defective derivative of Tn10, Tn10Δ16Δ17 (30). MudJ is an abbreviation for MudI1734, a Kanr mini-Mu derivative that forms stable lac operon fusions (31). MudA is an abbreviation for Mud1–8, an Ampr Mu derivative that forms stable lac operon fusions (32). (thr-pyrB substitution from S. typhimurium) refers to hybrid strains of S. typhi that contain a replacement of the thr-pyrB region of DNA from S. typhimurium. Genetic nomenclature is derived from the genetic map of S. typhimurium edition VIII (33).