Table 1

In vivo analysis of Cr−/− mice

ParameterCr+/+Cr−/−
Body weight at 6 weeks, g
 Male27  ±  426  ±  3
 Female22  ±  321  ±  3
Open field exploratory test
 Latency to move, s6  ±  35  ±  3
 Horizontal activity,*n81  ±  1583  ±  17
 Vertical activity,*n18  ±  516  ±  6
Hot plate test
 Latency to escape, s22  ±  725  ±  10
Rotorod (retention time), s150  ±  30135  ±  37
  • * In the open field exploratory test, Cr+/+ (n = 10) and Cr−/− mice (n = 10) were placed in an arena divided in 16 equal squares. The number of squares crossed over a 3.5-min period was recorded as horizontal activity. The number of rearing events over the same period was recorded as vertical activity. Values are the means ± SEM. 

  • Cr+/+ (n = 7) and Cr−/− mice (n = 7) were placed on a plate heated at 55°C, and the latency to escape the plate was recorded. The values are the means ± SEM. 

  • The rotating rod task was carried out for 5 min and the retention time on the rotating rod was counted for Cr+/+ (n = 8) and Cr−/− mice (n = 8). Five trials per day were given for 2 consecutive days; results are the means ± SEM for the second day.