Table 1

Sterol accumulation profiles of wild-type and ergosterol-deficient strains

Yeast genotypeSterols accumulated
WA1 (ERG11 ERG25 SLU1)Ergosterol (65), zymosterol (6), fecosterol (9), episterol (9), lanosterol (5), 4,4-dimethylzymosterol (3)
erg25(*)4,4-Dimethylzymosterol (100)
erg11(*)Lanosterol (86), 14-methylfecosterol (7), obtusifoliol (7)
erg11 erg25(*)Lanosterol (94), 24-methylenelanosterol (6)
slu1 Ergosta 5,7-dien-3β-ol (65), ergosterol (18), lanosterol (8), zymosterol (5), 14-methylfecosterol (3), fecosterol (1)
slu2 Lanosterol (45), fecosterol (or ergosta-5,7-dien-3β-ol) (32), obtusifoliol (5), 14-methylfecosterol (15), ergosterol (3)
erg25 slu1(*)Lanosterol (22), 4,4-dimethylzymosterol (78)
erg25 slu2 (*)Lanosterol (42), 4,4-dimethylzymosterol (58)
erg11 erg25 slu1 Lanosterol (92), 24-methylenelanosterol (8)
erg11 erg25 slu2 Lanosterol (93), 24-methylenelanosterol (7)
  • Sterols accumulating in each strain are listed with percentage of each sterol in parentheses. Only the wild-type slu1, slu2, and erg11 erg25 slu1 (slu2) strains were grown aerobically in YPD cultures. Sterol requiring strains (*) were grown in anaerobic jars on solid medium containing exogenous sterol.