Table 1

Effect of antibodies on organelle motility in bulk regions of extruded squid axoplasm.

Organelle velocity, μm/secMotile activity
Buffer2.0  ± 0.4, n = 28+
Anti-centractin
 UP-3852.1  ± 0.4, n = 21+
Anti-p150Glued
 UP-2350.6  ± 0.4, n = 5
 UP-235 (Fab)0.4  ± 0.1, n = 4
 UP-2361.0  ± 0.2, n = 10−/+
 UP-236 (Fab)0.9  ± 0.4, n = 11−/+
  • The central axoplasm was examined by VEC-DIC 60 min after extrusion into either motility buffer alone, buffer containing 0.8 mg/ml of affinity-purified antibody, or 0.5 mg/ml affinity-purified Fab fragments. +, >50 organelles moving per microscopic field; −/+, <10 organelles moving per microscopic field; −, <1 organelle moving per microscopic field.