Effect of SH3 domain-containing fusion proteins on dynamin GTPase activity

AdditionGTPase activity, mol/min per mg
None9.9 ± 2.0
GST17.0 ± 0.1
c-Src SH385.0 ± 12.4
GRB2131.9 ± 11.9
GRB2 SH3 (N)16.4 ± 0.9
GRB2 SH3 (C)12.0 ± 1.4
Mts658.2 ± 8.3
  • Dynamin GTPase activity was assayed in the presence of either the indicated glutathione S-transferase (GST) fusion proteins (0.08 mg/ml) or Mts (0.12 mg/ml). Errors indicate the range of duplicate data points. N, N-terminal SH3 domain; C, C-terminal SH3 domain. [Reproduced with permission from Herskovits et al. (36) (Copyright 1993, Proc. Natl. Acad. Sci. USA).]