Rate constants for association (kon) and dissociation (koff) of the complex between IL-4 variants and IL4-BP immobilized at a biosensor matrix

IL-4 variantkon × 10−6 M−1·s−1koff × 103 s−1IL-4 variantkon × 10−6 M−1·s−1koff × 103 s−1
IL4132.1
I5A1214I5R148.7
T6A141.9T6D8.815
Q8A162.5Q8R111.9
E9Q8.7270E9KNDND
E9ANDND
I11A112
K12S121.9K12E7.31.5
T13A8.47.1T13D7.60.85
N15A152.3N15D121.7
S16A161.9S16D111.5
E19A181.7E19R121.6
R53Q117.3
K77A102.1K77E4.42
Q78A112.2Q78E112.7
R81A7.72.8R81E3.26.1
F82A152.1F82D120.73
K84A102.9K84D2.49.3
R85A8.12.7R85E3.64.6
R88Q7.3140R88DNDND
R88A8.3760
N89A1227N89RNDND
W91A116.1W91D5.88.5
  • The association and dissociation of IL-4 variants and IL4-BP immobilized at a biosensor matrix was measured in real time by means of a BIA2000 system. The rate constants represent mean values of 12–24 independent measurements performed with at least three different analyte concentrations. The mean standard deviation was 15% for the kon and 25% for the koff values. ND, not determined (only unspecific binding was found).