Kinetic parameters of purified point mutants of 37-kDa PTP1B assessed with pTyr-RCML as substrate

EnzymeVmax, nmol/min/mgKm, nMkcat, min−1
Wild type60,2001022244
Tyr-46 → Ser4,1201700154
→ Leu4,1601700155
Glu-115 → Ala5,70045212
→ Asp5,90020220
Lys-116 → Ala68,6001502557
Lys-120 → Ala19,00080708
Asp-181 → Ala0.61≤1260.023
→ Glu97103.6
His-214 → Ala7002026
Cys-215 → SerNo detectable activity
Arg-221 → Lys11800.41
→ Met3.310600.12
Gln-262 → Ala720927