Surface expression of CXCR4 is restricted to mononuclear cells

Leukocyte subsetCXCR4 expression, % positive*Specific fluorescence intensity of 12G5-positive cells, MESF
Neutrophils0.4  ±  0.3
Eosinophils0.0  ±  0.2
T lymphocytes (CD3+ cells)41.6  ±  16.13580  ±  1423
B lymphocytes (CD20+ cells)53.5  ±  18.44989  ±  2264
Natural killer cells (CD56+ cells)0.2  ±  0.3
Monocytes (CD14+ cells)20.4  ±  9.41525  ±  537
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  • The percentage of cells stained with control mAb was <3%, and it was subtracted. Results are mean ± SD of eight experiments. 

  • The fluorescence intensity (MESF, molecules of equivalent soluble fluorochrome) of leukocytes stained with a control mAb was 37 for lymphocytes and 1622 for monocytes, and these values were subtracted. Results are mean ± SD.