Production of plaque-forming units after virus infection of transfected NIH 3T3 cells

Plasmid*Virus yield, plaque-forming units
CVB3CVB4Poliovirus
pRTHR2.92  ×  1071.7  ×  1062.65  ×  104
pRTMR4.22  ×  1066.0  ×  1052.60  ×  104
pBK-CMV2.25  ×  1041.5  ×  1032.27  ×  104
  • Plasmids encoding HCAR (pRTHR), MCAR (pRTMR), and vector alone (pBK-CMV) were transfected into receptor-negative NIH 3T3 cells and assayed for virus production 24 h post-transfection with a multiplicity of infection of around 50. 

  • Virus titers of transfected cell supernatants were determined by plaque assay on HeLa cells 24 h postinfection as described (8).